trf2 (Novus Biologicals)
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trf2
Trf2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti-trf2/TRF-2+Antibody+-+BSA+Free/pmc13104688-455-6-7
Average 95 stars, based on 129 article reviews
Trf2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti-trf2/TRF-2+Antibody+-+BSA+Free/pmc13104688-455-6-7
Average 95 stars, based on 129 article reviews
trf2 - by Bioz Stars,
2026-10
95/100 stars
Images
1) Product Images from "Local heterochromatin enrichment promotes telomere clustering and PML nuclear body assembly at telomeres"
Article Title: Local heterochromatin enrichment promotes telomere clustering and PML nuclear body assembly at telomeres
Journal: Cell reports
doi: 10.1016/j.celrep.2026.117004
Figure Legend Snippet: (A) Schematic of the fusion-protein constructs. Top: full constructs contain a TetON doxycycline (DOX)-inducible promoter, a Myc-tagged-TRF1 fused to a chromatin modifier (here, KRAB), a T2A, and mCherry-NES (nuclear export signal). (B) Western blot showing expression of the constructs (anti-Myc antibody) and H3K9me3 global levels in U2OS after 3 days of DOX induction. (C) Representative chromatin immunoprecipitation of H3, H3K9me3, and HP1α at telomeres and ALU repeats in U2OS after 3 days of DOX induction (T-KRAB: TRF1-KRAB). (D) Quantification of (C). Data represent mean ± SEM of n = 3 independent biological replicates. (E) Representative images of IF-FISH assessing PML colocalization with telomeres (TTAGGG) in U2OS after 3 days of DOX induction. Scale bars, 10 μm. (F–I) Quantification of (E). (F) Number of telomere foci per cell. (G) Standard deviation of the telomere foci area within each cell. (H) Number of total (small and large) telomere-PML colocalizations (TPF). (I) percentage of cells with at least 5 APBs. (F–H) Data represent mean ± SEM of all cells analyzed over three biological replicates. n (number of cells analyzed) = 110 (WT), 115 (TRF1), and 113 (TRF1-KRAB). (I) Data represent mean ± SEM of n = 3 independent biological replicates, with at least 30 cells analyzed per replicate. (J) Representative C-circle assay in U2OS after 3 days of DOX induction. Right: quantification. Data represent mean ± SEM of n = 3 independent biological replicates. (K) G2 telomere synthesis: colocalization of TRF2 with G2-incorporated EdU in U2OS after 3 days of DOX induction. Scale bars, 10 μm. Right: quantification. Data represent mean ± SEM of 3 independent biological replicates, with at least 30 cells analyzed per replicate. Statistical analyses: for (D and F–I), ordinary one-way ANOVA. For (J and K), Welch’s t test. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. ns, non-significant. See also .
Techniques Used: Construct, Western Blot, Expressing, Chromatin Immunoprecipitation, Standard Deviation
Figure Legend Snippet: (A) Schematic of the fusion-protein constructs. Here, Myc-TRF1 is fused to HP1α V22M, a mutation that abrogates H3K9me3 binding. (B) Western blot showing the expression of the constructs (anti-Myc antibody) in U2OS after 3 days of DOX induction. (C)Representative chromatin immunoprecipitation of H3, H3K9me3, and HP1α at telomeres and ALU repeats in U2OS after 3 days of DOX induction (T-HP1α: TRF1-HP1α). (D) Quantification of (C). Data represent mean ± SEM of n = 3 independent biological replicates. (E) Representative images of IF-FISH assessing PML colocalization with telomeres (TTAGGG) in U2OS after 3 days of DOX induction. Scale bars, 10 μm. (F) Quantification of cells with at least 5 APBs from (E). Data represent mean ± SEM of n = 3 independent biological replicates, with at least 30 cells analyzed per replicate. (G) Representative C-circle assay in U2OS after 3 days of DOX induction. Right: quantification. Data represent mean ± SEM of n = 3 independent biological replicates. (H) G2 telomere synthesis: colocalization of TRF2 with G2-incorporated EdU in U2OS after 3 days of DOX induction. Left: representative images. Scale bars, 10 μm. Right: quantification. Data represent mean ± SEM of n = 3 independent biological replicates, with at least 30 cells analyzed per replicate. (I) Quantification from (E) of the standard deviation between telomeric foci area within each cell. Data represent mean ± SEM of all cells from 3 independent biological replicates. n (number of cells analyzed) = 110 (WT), 115 (TRF1), and 100 (TRF1-HP1α). (J) Representative image from experiment in (E) of a cell with ultra-bright APBs (left) and cells with a telomeric bridge (right) in U2OS + TRF1-HP1α. Scale bar: 10 μm. (K and L) Percentage of cells with ultra-bright APBs (K) and entanglements (L). Data represent mean ± SEM of 3 independent biological replicates, with at least 30 cells analyzed per replicate. Statistical analyses: for (D, F, I, and K–L), ordinary one-way ANOVA. For (G and H), Welch’s t test. * p < 0.05, ** p < 0.01, and ** p < 0.0001. ns, non-significant. See also .
Techniques Used: Construct, Mutagenesis, Binding Assay, Western Blot, Expressing, Chromatin Immunoprecipitation, Standard Deviation
Figure Legend Snippet: (A) Quantification of basal levels of telomere-PML colocalization (TPF) in HeLa ST and HeLa LT. Data represent mean ± SEM of 3 independent biological replicates. (B) Western blot showing siRNA-mediated knockdown of HP1α in HeLa LT control cells and expressing TRF1-KRAB (T-KRAB). (C) Western blot showing Myc (TRF1-KRAB), HP1α, H3K9me3, and actin in indicated cell lines. (D) Quantification of TPF in indicated cell lines. Data represent mean ± SEM of n = 3 independent biological replicates, with at least 30 cells analyzed per replicate. (E) Representative images of IF-FISH assessing PML (IF) colocalization with telomeres (TTAGGG) in HeLa LT with indicated constructs after 3 days of DOX induction. Scale bars, 10 μm. (F) Quantification of TPF from (E). Data represent mean ± SEM of n = 3 independent biological replicates, with at least 30 cells analyzed per replicate. (G) C-circle assay in HeLa LT after 3 days of DOX induction. Data represent mean ± SEM of n = 3 independent biological replicates. (H) G2 telomere synthesis: colocalization of TRF2 with G2-incorporated EdU in HeLa LT after 3 days of DOX induction. Scale bars, 10 μm. (I) Quantification of (H). Data represent mean ± SEM of n = 3 independent biological replicates, with at least 30 cells analyzed per replicate. (J) Representative image and quantification of entanglements in HeLa LT with indicated constructs. Data represent mean ± SEM of n = 3 independent biological replicates, with at least 30 cells analyzed per replicate. Scale bar: 10 μm. (K) Representative images of IF-FISH assessing PML (IF) colocalization with telomeres (TTAGGG) in HeLa ST with indicated constructs after 3 days of DOX induction. Scale bars, 10 μm. (L and M) Quantification of TPF (L) and entanglements (M) from (K). Data represent mean ± SEM of n = 3 independent biological replicates, with at least 30 cells analyzed per replicate. Statistical analyses: for (B, D, F, I, and L), ordinary one-way ANOVA. For (G, J, and M), Welch’s t test. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. ns, non-significant. See also .
Techniques Used: Western Blot, Knockdown, Control, Expressing, Construct
Related Articles
Western Blot:Article Title: RECQL4, the Protein Mutated in Rothmund-Thomson Syndrome, Functions in Telomere Maintenance Article Snippet: For in vivo co-IP of TRF2 by RECQL4, U2OS cells were lysed in cell lysis buffer (50 m m Tris-HCl, pH 7.4, 150 m m NaCl, 1 m m EDTA, 1% Triton X-100, and 1× protease inhibitors (Roche Applied Science)), and co-IP was performed as described elsewhere using Article Title: Transcription regulation of CDKN1A (p21/CIP1/WAF1) by TRF2 is epigenetically controlled through the REST repressor complex Article Snippet: After blocking the membrane was incubated with primary Article Title: SIRT6 is required for maintenance of telomere position effect in human cells Article Snippet: The antibodies used were as follows: anti-SIRT6 (ref , 0.4 μg/ml), anti-SIRT1 (Santa Cruz, 0.1μg/ml), anti-β-tubulin (Millipore, 0.2 μg/ml), anti-H3K9Ac (Sigma, 1.2 μg per ChIP), anti-H3K9me3 (Abcam, 1.2 μg per ChIP), anti-H3 (Abcam, 1.2 μg per ChIP), Article Title: Tousled-Like Kinases Suppress Innate Immune Signaling Triggered by Alternative Lengthening of Telomeres Article Snippet: Rabbit polyclonal anti-TRF2 , Article Title: Low doses of nanodiamonds and silica nanoparticles have beneficial hormetic effects in normal human skin fibroblasts in culture. Article Snippet: Nanodiamonds (ND) and silica nanoparticles (SiO2-NP) have been much investigated for their toxicity at high doses, little is known about their biological activity at low concentrations.. Here we report the biphasic dose response of ND and SiO2-NP in modulating normal human facial skin fibroblasts (FSF1) in culture.. ND and SiO2-NP at low concentration (up to 0.5 mg/ml) had beneficial effects on FSF1 in terms of increasing their proliferation and metabolic activity. Article Title: Telomerase promotes formation of a telomere protective complex in cancer cells Article Snippet: The following were the antibodies used: anti-Hsp70/Hsp72 2H9 (mouse monoclonal, catalog no. MABE1130, 1:200; Merck Millipore), Article Title: WRN Loss Induces Switching of Telomerase-Independent Mechanisms of Telomere Elongation Article Snippet: Primary antibodies used were: anti-WRN (Abcam), anti-BLM (Bethyl Laboratories), anti-lamin B (Santa Cruz), Article Title: Telomeres control human telomerase ( TERT ) expression through non-telomeric TRF2 Article Snippet: Blocking Assay:Article Title: RECQL4, the Protein Mutated in Rothmund-Thomson Syndrome, Functions in Telomere Maintenance Article Snippet: For in vivo co-IP of TRF2 by RECQL4, U2OS cells were lysed in cell lysis buffer (50 m m Tris-HCl, pH 7.4, 150 m m NaCl, 1 m m EDTA, 1% Triton X-100, and 1× protease inhibitors (Roche Applied Science)), and co-IP was performed as described elsewhere using Article Title: Transcription regulation of CDKN1A (p21/CIP1/WAF1) by TRF2 is epigenetically controlled through the REST repressor complex Article Snippet: After blocking the membrane was incubated with primary Article Title: SIRT6 is required for maintenance of telomere position effect in human cells Article Snippet: The antibodies used were as follows: anti-SIRT6 (ref , 0.4 μg/ml), anti-SIRT1 (Santa Cruz, 0.1μg/ml), anti-β-tubulin (Millipore, 0.2 μg/ml), anti-H3K9Ac (Sigma, 1.2 μg per ChIP), anti-H3K9me3 (Abcam, 1.2 μg per ChIP), anti-H3 (Abcam, 1.2 μg per ChIP), Article Title: Tousled-Like Kinases Suppress Innate Immune Signaling Triggered by Alternative Lengthening of Telomeres Article Snippet: Rabbit polyclonal anti-TRF2 , Article Title: Low doses of nanodiamonds and silica nanoparticles have beneficial hormetic effects in normal human skin fibroblasts in culture. Article Snippet: Nanodiamonds (ND) and silica nanoparticles (SiO2-NP) have been much investigated for their toxicity at high doses, little is known about their biological activity at low concentrations.. Here we report the biphasic dose response of ND and SiO2-NP in modulating normal human facial skin fibroblasts (FSF1) in culture.. ND and SiO2-NP at low concentration (up to 0.5 mg/ml) had beneficial effects on FSF1 in terms of increasing their proliferation and metabolic activity. Article Title: Telomerase promotes formation of a telomere protective complex in cancer cells Article Snippet: The following were the antibodies used: anti-Hsp70/Hsp72 2H9 (mouse monoclonal, catalog no. MABE1130, 1:200; Merck Millipore), Article Title: WRN Loss Induces Switching of Telomerase-Independent Mechanisms of Telomere Elongation Article Snippet: Primary antibodies used were: anti-WRN (Abcam), anti-BLM (Bethyl Laboratories), anti-lamin B (Santa Cruz), Article Title: Telomeres control human telomerase ( TERT ) expression through non-telomeric TRF2 Article Snippet: Membrane:Article Title: RECQL4, the Protein Mutated in Rothmund-Thomson Syndrome, Functions in Telomere Maintenance Article Snippet: For in vivo co-IP of TRF2 by RECQL4, U2OS cells were lysed in cell lysis buffer (50 m m Tris-HCl, pH 7.4, 150 m m NaCl, 1 m m EDTA, 1% Triton X-100, and 1× protease inhibitors (Roche Applied Science)), and co-IP was performed as described elsewhere using Article Title: Transcription regulation of CDKN1A (p21/CIP1/WAF1) by TRF2 is epigenetically controlled through the REST repressor complex Article Snippet: After blocking the membrane was incubated with primary Article Title: SIRT6 is required for maintenance of telomere position effect in human cells Article Snippet: The antibodies used were as follows: anti-SIRT6 (ref , 0.4 μg/ml), anti-SIRT1 (Santa Cruz, 0.1μg/ml), anti-β-tubulin (Millipore, 0.2 μg/ml), anti-H3K9Ac (Sigma, 1.2 μg per ChIP), anti-H3K9me3 (Abcam, 1.2 μg per ChIP), anti-H3 (Abcam, 1.2 μg per ChIP), Article Title: Tousled-Like Kinases Suppress Innate Immune Signaling Triggered by Alternative Lengthening of Telomeres Article Snippet: Rabbit polyclonal anti-TRF2 , Article Title: Low doses of nanodiamonds and silica nanoparticles have beneficial hormetic effects in normal human skin fibroblasts in culture. Article Snippet: Nanodiamonds (ND) and silica nanoparticles (SiO2-NP) have been much investigated for their toxicity at high doses, little is known about their biological activity at low concentrations.. Here we report the biphasic dose response of ND and SiO2-NP in modulating normal human facial skin fibroblasts (FSF1) in culture.. ND and SiO2-NP at low concentration (up to 0.5 mg/ml) had beneficial effects on FSF1 in terms of increasing their proliferation and metabolic activity. Article Title: Telomerase promotes formation of a telomere protective complex in cancer cells Article Snippet: The following were the antibodies used: anti-Hsp70/Hsp72 2H9 (mouse monoclonal, catalog no. MABE1130, 1:200; Merck Millipore), Article Title: WRN Loss Induces Switching of Telomerase-Independent Mechanisms of Telomere Elongation Article Snippet: Primary antibodies used were: anti-WRN (Abcam), anti-BLM (Bethyl Laboratories), anti-lamin B (Santa Cruz), Article Title: Telomeres control human telomerase ( TERT ) expression through non-telomeric TRF2 Article Snippet: Incubation:Article Title: RECQL4, the Protein Mutated in Rothmund-Thomson Syndrome, Functions in Telomere Maintenance Article Snippet: For in vivo co-IP of TRF2 by RECQL4, U2OS cells were lysed in cell lysis buffer (50 m m Tris-HCl, pH 7.4, 150 m m NaCl, 1 m m EDTA, 1% Triton X-100, and 1× protease inhibitors (Roche Applied Science)), and co-IP was performed as described elsewhere using Article Title: Transcription regulation of CDKN1A (p21/CIP1/WAF1) by TRF2 is epigenetically controlled through the REST repressor complex Article Snippet: After blocking the membrane was incubated with primary Article Title: SIRT6 is required for maintenance of telomere position effect in human cells Article Snippet: The antibodies used were as follows: anti-SIRT6 (ref , 0.4 μg/ml), anti-SIRT1 (Santa Cruz, 0.1μg/ml), anti-β-tubulin (Millipore, 0.2 μg/ml), anti-H3K9Ac (Sigma, 1.2 μg per ChIP), anti-H3K9me3 (Abcam, 1.2 μg per ChIP), anti-H3 (Abcam, 1.2 μg per ChIP), Article Title: Tousled-Like Kinases Suppress Innate Immune Signaling Triggered by Alternative Lengthening of Telomeres Article Snippet: Rabbit polyclonal anti-TRF2 , Article Title: Low doses of nanodiamonds and silica nanoparticles have beneficial hormetic effects in normal human skin fibroblasts in culture. Article Snippet: Nanodiamonds (ND) and silica nanoparticles (SiO2-NP) have been much investigated for their toxicity at high doses, little is known about their biological activity at low concentrations.. Here we report the biphasic dose response of ND and SiO2-NP in modulating normal human facial skin fibroblasts (FSF1) in culture.. ND and SiO2-NP at low concentration (up to 0.5 mg/ml) had beneficial effects on FSF1 in terms of increasing their proliferation and metabolic activity. Article Title: Telomerase promotes formation of a telomere protective complex in cancer cells Article Snippet: The following were the antibodies used: anti-Hsp70/Hsp72 2H9 (mouse monoclonal, catalog no. MABE1130, 1:200; Merck Millipore), Article Title: WRN Loss Induces Switching of Telomerase-Independent Mechanisms of Telomere Elongation Article Snippet: Primary antibodies used were: anti-WRN (Abcam), anti-BLM (Bethyl Laboratories), anti-lamin B (Santa Cruz), Article Title: Telomeres control human telomerase ( TERT ) expression through non-telomeric TRF2 Article Snippet: In Vivo:Article Title: RECQL4, the Protein Mutated in Rothmund-Thomson Syndrome, Functions in Telomere Maintenance Article Snippet: For in vivo co-IP of TRF2 by RECQL4, U2OS cells were lysed in cell lysis buffer (50 m m Tris-HCl, pH 7.4, 150 m m NaCl, 1 m m EDTA, 1% Triton X-100, and 1× protease inhibitors (Roche Applied Science)), and co-IP was performed as described elsewhere using Article Title: Transcription regulation of CDKN1A (p21/CIP1/WAF1) by TRF2 is epigenetically controlled through the REST repressor complex Article Snippet: After blocking the membrane was incubated with primary Article Title: SIRT6 is required for maintenance of telomere position effect in human cells Article Snippet: The antibodies used were as follows: anti-SIRT6 (ref , 0.4 μg/ml), anti-SIRT1 (Santa Cruz, 0.1μg/ml), anti-β-tubulin (Millipore, 0.2 μg/ml), anti-H3K9Ac (Sigma, 1.2 μg per ChIP), anti-H3K9me3 (Abcam, 1.2 μg per ChIP), anti-H3 (Abcam, 1.2 μg per ChIP), Article Title: Tousled-Like Kinases Suppress Innate Immune Signaling Triggered by Alternative Lengthening of Telomeres Article Snippet: Rabbit polyclonal anti-TRF2 , Article Title: Low doses of nanodiamonds and silica nanoparticles have beneficial hormetic effects in normal human skin fibroblasts in culture. Article Snippet: Nanodiamonds (ND) and silica nanoparticles (SiO2-NP) have been much investigated for their toxicity at high doses, little is known about their biological activity at low concentrations.. Here we report the biphasic dose response of ND and SiO2-NP in modulating normal human facial skin fibroblasts (FSF1) in culture.. ND and SiO2-NP at low concentration (up to 0.5 mg/ml) had beneficial effects on FSF1 in terms of increasing their proliferation and metabolic activity. Article Title: Telomerase promotes formation of a telomere protective complex in cancer cells Article Snippet: The following were the antibodies used: anti-Hsp70/Hsp72 2H9 (mouse monoclonal, catalog no. MABE1130, 1:200; Merck Millipore), Article Title: WRN Loss Induces Switching of Telomerase-Independent Mechanisms of Telomere Elongation Article Snippet: Primary antibodies used were: anti-WRN (Abcam), anti-BLM (Bethyl Laboratories), anti-lamin B (Santa Cruz), Article Title: Telomeres control human telomerase ( TERT ) expression through non-telomeric TRF2 Article Snippet: Lysis:Article Title: RECQL4, the Protein Mutated in Rothmund-Thomson Syndrome, Functions in Telomere Maintenance Article Snippet: For in vivo co-IP of TRF2 by RECQL4, U2OS cells were lysed in cell lysis buffer (50 m m Tris-HCl, pH 7.4, 150 m m NaCl, 1 m m EDTA, 1% Triton X-100, and 1× protease inhibitors (Roche Applied Science)), and co-IP was performed as described elsewhere using Article Title: Transcription regulation of CDKN1A (p21/CIP1/WAF1) by TRF2 is epigenetically controlled through the REST repressor complex Article Snippet: After blocking the membrane was incubated with primary Article Title: SIRT6 is required for maintenance of telomere position effect in human cells Article Snippet: The antibodies used were as follows: anti-SIRT6 (ref , 0.4 μg/ml), anti-SIRT1 (Santa Cruz, 0.1μg/ml), anti-β-tubulin (Millipore, 0.2 μg/ml), anti-H3K9Ac (Sigma, 1.2 μg per ChIP), anti-H3K9me3 (Abcam, 1.2 μg per ChIP), anti-H3 (Abcam, 1.2 μg per ChIP), Article Title: Tousled-Like Kinases Suppress Innate Immune Signaling Triggered by Alternative Lengthening of Telomeres Article Snippet: Rabbit polyclonal anti-TRF2 , Article Title: Low doses of nanodiamonds and silica nanoparticles have beneficial hormetic effects in normal human skin fibroblasts in culture. Article Snippet: Nanodiamonds (ND) and silica nanoparticles (SiO2-NP) have been much investigated for their toxicity at high doses, little is known about their biological activity at low concentrations.. Here we report the biphasic dose response of ND and SiO2-NP in modulating normal human facial skin fibroblasts (FSF1) in culture.. ND and SiO2-NP at low concentration (up to 0.5 mg/ml) had beneficial effects on FSF1 in terms of increasing their proliferation and metabolic activity. Article Title: Telomerase promotes formation of a telomere protective complex in cancer cells Article Snippet: The following were the antibodies used: anti-Hsp70/Hsp72 2H9 (mouse monoclonal, catalog no. MABE1130, 1:200; Merck Millipore), Article Title: WRN Loss Induces Switching of Telomerase-Independent Mechanisms of Telomere Elongation Article Snippet: Primary antibodies used were: anti-WRN (Abcam), anti-BLM (Bethyl Laboratories), anti-lamin B (Santa Cruz), Article Title: Telomeres control human telomerase ( TERT ) expression through non-telomeric TRF2 Article Snippet: |
